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FluoTags are camelid single domain antibodies consisting only of one antigen binding site of a Alpaka heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.
|Cat. No. N1602-At565-L||
200µl 5µM camelid sdAb in buffered saline, 50% glycerol, 0.09% sodium azide
WB: not recommended
ICC: 1 : 500
IHC: 1 : 500
IHC-P/FFPE: not tested yet
|Label||ATTO 565, two fluorophores coupled to one FluoTag|
|Immunogen||Recombinant protein corresponding to AA 58 to 515 from rat VGLUT1 (UniProt Id: Q62634)|
Epitop: AA 58 to 515 from rat VGLUT1 (UniProt Id: Q62634)
Reacts with: rat (Q62634), mouse (Q3TXX4).
Other species not tested yet.
|Specificity||Specific for VGLUT 1|
The vesicular glutamate transporter 1 VGLUT 1, also referred to as BNPI and SLC17A7, was originally identified as a brain specific phosphate transporter. Like the related VGLUT 2, VGLUT 1 is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.
In FluoTag-X two fluorophore molecules are site-specifically coupled to each FluoTag molecule. Owing to the small size of the FluoTags, the distance between the target epitope and each fluorophore is below 4 nm.
In comparison to detection systems using conventional antibodies, FluoTag-X can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render the FluoTag-X products excellent tools for all microscopy techniques.