Synaptic Systems - Delta2-tubulin

Cat. No.: 302 213

Amount: 50 µg

Price: $380.00

WB

ICC

IHC

IHC-P

Background

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintainance of cell shape.
Tubulin itself is a globular protein which consists of two polypeptides, α-tubulin and β-tubulin. α- and β-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase ads a C-terminal tyrosin to monomeric α-tubulin.
Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated α-tubulin is referred to as Glu-α-tubulin. Another post-translational modification of detyrosinated α-tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle. A third variant of detyrosinated α-tubulin is Δ2-tubulin which lacks the C-terminal glutamic acid. It cannot be tyrosinated by tyrosine ligase and is one of the dominant α-tubulin isoforms in neurons.

1 ) Erck C et al., Proc. Natl. Acad. Sci. U.S.A. (2005)
2 ) Contin MA et al., Biochem. J. (1999)
3 ) Paturle-Lafanechère L et al., J. Cell. Sci. (1994)
4 ) Ersfeld K et al., J. Cell Biol. (1993)
5 ) Rüdiger M et al., FEBS Lett. (1992)
6 ) Caron JM et al., J. Cell Biol. (1985)
7 ) Caron JM et al., Nature ()

Protocols

Cat. No. 302 213	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 200 up to 1 : 500 (see remarks) gallery IHC: yes gallery IHC-P: 1 : 500 gallery
Immunogen	Synthetic peptide corresponding to AA 443 to 449 from rat Δ2-tubulin (UniProt Id: P68370)
Reactivity	Reacts with: human, rat (P68370), mouse, mammals. Other species not tested yet.
Specificity	Specific for Δ2-tubulin.
Remarks	This antibody has been cross-adsorbed against peptides corresponding to the C-termini of glu- and tyr-tubulin. ICC: Requires long permeabilization with 0.3% Triton X 100 for 30 min.
Data sheet	302_213.pdf

Delta2-tubulin antibody - 302 213